Products

DNA Polymerase Assay - DNA synthesis assay

DNA polymerase III synthesizes DNA using the RNA primer made by the DNA primase at the DNA replication

fork of bacteria.   DNA polymerase III alpha is the catalytic subunit of the polymerase. The DNA Polymerase

Assay Kit is based on measurement of the DNA molecules synthesized by the DNA polymerase. The assay

can be performed in 96-well plate or 384-well plate format for high throughput screening of DNA polymerase

inhibitors.   

DNA Polymerase Assay Kit (enzyme not included)

Each kit (Catalog number DPA100K) includes the assay reagents except the enzyme for 100 assays of DNA

polymerase reactions in a 384-well assay format.  The assay conditions are optimized for bacterial DNA

polymerase III alpha subunit.  

E. coli DNA Polymerase Assay Kit Plus (enzyme included)

The E. coli DNA Polymerase Assay Kit Plus includes the assay buffer, E. coli DNA polymerase III alpha

subunit and DNA template for 100 assays of DNA polymerase reactions in a 384-well assay format.

H. influenzae DNA Polymerase Assay Kit Plus (enzyme included)

Each kit includes the assay buffer and DNA template for 100 assays of DNA polymerase reactions in

a 384-well assay format.  H. influenzae DNA polymerase III alpha is included.

S. pneuminiae DNA Polymerase Assay Kit Plus (enzyme included)

Each kit includes the assay buffer and DNA template for 100 assays of DNA polymerase reactions in

a 384-well assay format.  S. pneumoniae DNA polymerase III alpha is included.

Reference:

Hou Z et al, Selective in vivo and in vitro activities of 3,3'-4-nitrobenzylidene-bis-4-hydroxycoumarin against

methicillin-resistant Staphylococcus aureus by inhibition of DNA polymerase III. Sci Rep. 2015 Sep 1;5:13637.