Products

Product

488/565/594/647-Phalloidin (Atto 488/565/594/647 phalloidin)

Origin

Amanita Phalloides Conjugate

Molecular mass

N/A

Product description

Rapid staining of single actin filaments with highly photostable 594-Phalloidin.

Phalloidin is a bicyclic peptide of a family of toxins isolated from the Amanita

phalloides “death cap” mushroom. The peptide is commonly used as a selective

stain or counterstain to specifically label F-actin in fixed or permeabilized cells, and

cell-free experiments. Labeled phalloidin conjugates bind in a stoichiometric ratio of

about one phallotoxin per actin subunit in both muscle and non-muscle cells, and

have similar affinity for both large and small filaments. Phalloidins do not bind to

monomeric actin and have a stabilizing effect on F-actin.

* 488 provides green fluorescence of unparalleled brightness and photostability

* 565-Phalloidin has a strong pink fluorescence of high brightness and superior

  photostability.

* 594/647-Phalloidin has a strong orange-red fluorescence of high brightness and

  superior photostability.

Actin interaction

Selective staining of actin filments in vitro.

Form

Dry

Quantity

1 x 100 Units

Buffer

Reconstitute in 100µl MeOH, DMSO or DMF. Dilute 5µl of the phalloidin stock with

500µl PBS and apply to stain  the sample.

Sub cellular

localization

Actin, Cytoskeleton

Excitation/Emission

* 488-Phalloidin; ex 488 nm, em 523 nm

* 565-Phalloidin; ex 563 nm, em 592 nm

* 594-Phalloidin; ex 601 nm, em 627 nm

* 594-Phalloidin; ex 647 nm, em 669 nm

Kit Content

1 x 100 Units 594-Phalloidin

1 x PolyMix (10 x conc., F-actin buffer, lyophilized)

Storage instructions

Upon reconstitution the product is stored at -5 to -70°C. Always protect from light.

Shipping conditions

At ambient temperature. Upon delivery store at -5 to -70°C.