Products

Protein Analysis Reagents_SDS-Remover & others

 Minute™ Non-Denatured Protein Solubilization Reagent 

This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat#

Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized

proteins are non-denatured and can be used for ELISA, immunoprecipitation/Co-IP, enzymatic activity

determination and other applications.

  Instruction sheet (PDF)

 Minute™ Protein Solubilization Reagent for MS 

This reagent is formulated for dissolving protein pellets using protein kits from Invent Biotechnologies (Cat#

Nos. SM-005, SM-005-P, CP-011, NE-013, YM-017, MM-018 and AF-023) and other companies. Solubilized

proteins are denatured due to the presence of SDS and can be used for trypsin digestion and for subsequent

mass spectrometry analysis.

  Instruction sheet (PDF)

 Minute™ SDS-Remover 

Sodium dodecyl sulfate (SDS) is one of the most commonly used detergents for biological research.

However, the presence of SDS in the sample could interfere with downstream applications. For instance,

SDS must be reduced to below certain concentration before trypsin digestion of protein can be performed

effectively. The presence of SDS in protein sample could interfere with mass/MS analysis and needs to be

removed prior to the analysis. The presence of SDS in protein samples also interfereswith antigen-antibody

binding and has a negative impact for experiments such as immunoprecipitation and ELISA. This proprietary

formulated reagent is designed for easy and rapid removal of SDS in solution by precipitation. The efficacy is

superior to SDS-removal column and TCA precipitation with minimum protein loss (<20%). Due to small

volume of SDS-Remover used, the final protein concentration of the sample is not significantly impacted.

  Instruction sheet (PDF)