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Carboxyl-functionalized Gold Nanoparticles

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Carboxyl-functionalized Gold Nanoparticles

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This product has been developed to facilitate conjugating gold nanoparticles to molecules that cannot be

conjugated via the classic direct adsorption method. The product is especially suited for covalent conjugation

of small ligands.

Aurion Gold Nanoparticles-Carboxyl Functionalized- are polyethylene glycol-carboxyl stabilized gold

nanoparticle solutions based on defined particle sizes of 6, 10, 15 and 25nm. They are prepared using in-

house developed production protocols giving the product the well known Aurion quality: narrow size

distribution combined with long lasting functionalization. Biomolecules with a primary amine available can

becovalently conjugated using well established EDC/sulfo-NHS chemistry.

Features:

• Carboxyl (-COOH) functionalized

• Particle sizes 6-10-15 and 25nm

• Functionalized and activated particles bind to primary amines in the target molecule

• Conjugation using EDC/sulfo-NHS chemistry

• Recommended for conjugation of small ligands

• Detailed conjugation protocol

• Ready to use, long term stability

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Biomolecules that are too small to be conjugated to Gold

Nanoparticles via adsorption can be covalently linked to Carboxyl-

functionalized Gold Nanoparticles. Primary amines are present in

e.g. the N-terminal side of peptides and in the side group of the

amino acid lysine.

The conjugation relies on well known and proven EDC/sulfo-NHS

chemistry.

EDC (N-(3-Dimethylaminopropyl)-N’-ethylcarbodiimide

hydrochloride) is a water soluble carbodiimide which transforms the

carboxyl groups on the gold to an active ester in the presence of

sulfo-NHS (N-Hydroxysulfosucciimide, sodium salt). These sulfo-

NHS esters are relatively stable in acidic environment and couple

rapidly to the amine(s) in the target molecules.

Aurion Gold Nanoparticles - Carboxyl Functionalized -have a

guaranteed shelf life of 12 months from the date of quality control

analysis.

The products should be stored at 4-8℃.

Freezing is not recommended.

Aurion Gold Nanoparticles - Carboxyl Functionalized - are supplied

in 10mM MES buffer, pH 5.0.

Gold Nanoparticle conjugation - 'Adsorption or Covalent Binding?' (PDF)

Ordering informations

Catalog No.	Product Name	Size
406.133	Aurion Gold Nanoparticles 6 nm	4 x 5ml at OD520nm = 1.0
410.133	Aurion Gold Nanoparticles 10 nm
415.133	Aurion Gold Nanoparticles 15 nm
425.133	Aurion Gold Nanoparticles 25 nm

▣ 관련 페이지 ; Aurion

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