Products

Golgi apparatus, also called Golgi complex or Golgi body, is made up of a series of flattened stacked

pouches (cisternae). Golgi apparatus is a vital organelle in eukaryotic cells and is responsible for

transporting, modifying, and packaging protein and lipids into vesicles for delivery to targeted locations.  The

quantity and distribution of Golgi in different cell and tissue types vary significantly. The ability to obtain

highly-enriched Golgi fraction is an important first step forstudying its function and interaction with other

organelles. Traditional methods for isolating Golgi apparatus are based on density gradient

ultracentrifugation. The protocol requires a large amount of starting material and the methods are tedious and

time-consuming. Unlike any other Golgi isolation kit, this kit uses a patented spin-column-based technology

that is simple, rapid and requires only a small amount of starting material. This kit can preferentially enrich

native Golgi by precipitation without usinga Dounce homogenizer and ultracentrifugation. The whole protocol

can be completed in < 2 hours.

Isolation of Golgi fraction from mouse liver tissue.  A. Ponceau S stained blot.  B. Wester blot. Lane 1. Total

liver lysate, 2. Frozen liver tissue, 3. Fresh liver tissue.  Organelle markers; Plasma membrane (Na/K

ATPase, Novus, NB300146S); Golgi (Novus NBP1-32793); Mitochondria (abcam, ab96333); GAPDH

(Biolegend, 631401).