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The kit is designed for rapid fractionation of fresh/frozen soft plant tissues, especially leaves. Unlike other

commercial kit and Lab protocols, of which large amount of starting material (a few grams) and lengthy

processing time are required, this kit only uses 100-200 mg starting material.  By using a specialized filter

cartridge coupled with our proprietary buffer system, four subcellular fractions (cytosol,nuclei, chloroplasts

and organelles) can be obtained in about 1 hour. Thesubcellular fractions can be used in numerous

applications that include but not limited to protein trafficking, nuclear protein extraction and nucleic acid

purification/sequencing. The highly enriched nuclei can be used for proteomics, flow cytometry analysis,

DNA sequencing, and protein-protein/protein-DNA interaction studies. The subcellular fractionation protocol

includes grinding tissue to release plant cells followed by disruption of the cell membrane by rapidly passing

through the filter. Different subcellularfraction is obtained by differential centrifugation using only a table top

centrifuge. This kit has been tested for use with many species such as Arabidopsis, Spinach, Peas, and

Rape.