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Isolated nuclei are widely used for a variety of experiments such as FACS , single nucleus analysis (such

as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle and apoptosis research. Single cell

RNA-seq is a powerful technology for studying complex cellular composition of tissues. However, Neurons

are highly interconnected and it is very difficult to obtain single cells from neuronal tissues such as brain and

spinal cord. It is even more difficult to isolate intact cells from frozenneuronal tissues. Due to these

limitations, single cell RNA-seq is being substituted by single nucleus-seq. Traditional method for single

nucleus isolation from neuronal tissue is relatively tedious and time consuming and the yield is usually low

because it is difficult to get rid of contaminating myelin and other cellular components. This kit is specially

designed to address the issues with a protocol that is simple, rapid and effective. Highly purified single

nucleus can be obtained in about 30 min. In comparisonto traditional method, the kit requires much less

starting material and can handle a large range of sample size (1-25 mg). The buffers contain proprietary mix

of detergents for efficient cell lysis. If the presence of detergent is not desirable, a detergent-free nuclei

isolation kit is also available under cat# NI-024.