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Isolation and purification of nuclei from tissues and cultured cells are common lab practice. Isolated nuclei,

especially single nucleus, are widely used for a variety of experiments such as FACS analysis, single

nucleus analysis (such as RNA-seq and ATAC-seq), immunofluorescence staining, cell cycle analysis and

apoptosis research. Traditional method for isolation of nuclei is relatively simple and straight forward.

However, nucleus aggregation and formation of high percentage of doubletsare frequently encountered

problems. This kit is specially designed to address these issues. The protocol is simple, rapid and very

effective. High percentage of well separated single nucleus can be obtained in about 30 min. In comparison

to traditional method the kit requires less starting material and has a larger range of sample size (1-30 mg).

The buffers contain proprietary mix of detergents for efficient cell lysis. If the presence of detergent is not

desired, a detergent-free nuclei isolation kit is alsoavailable under cat# NI-024