Products

CircAID-p-seq for Oxford Nanopore Technologies

CircAID-p-seq kit is designed for quick (1-day) high-quality library preparation for short RNAs bearing a 3’-

phosphate/2’,3’-cyclic phosphate compatible with Oxford Nanopore platform.

Features:

• Selective sequencing of short RNAs bearing a 3’P  or 2′,3′ cP end

• Quick (1-day) high quality library preparation

• No library PCR amplification bias

• Suitable for RNA footprinting, Ribosome Profiling and transcriptome analysis.

CircAID-p-seq kit uses our proprietary CircAID-p-seq (Circular Amplification and IDentification of short RNA

sequences bearing a 3′ Phosphate) technology, to generate Oxford Nanopore-compatible sequencing

libraries without need for PCR amplification. The kit is designed for quick (1-day) high quality library

preparation for short RNAs (20-50 nt) bearing a 3’-phosphate/2’,3’-cyclic phosphate (3’-P/2’,3’-cP) end.

CircAID-p-seq (catalog no #CA001) includes all the components necessary for(i) first adaptor ligation, (ii)

circularization, (iii) Reverse Transcription – Rolling Circle Amplification (RT-RCA). The final library product is

suitable for next-generation sequencing (NGS) on the Oxford Nanopore platform, thus enabling real-time

single-molecule detection of biologically relevant RNA species.

Applications:

• RNA footprinting

• Ribosome profiling

• Transcriptome analysis

CircAID-p-seq includes all the components necessary for (i) first adaptor ligation, (ii) circularization, (iii)

Reverse Transcription – Rolling Circle Amplification (RT-RCA). The final library product is suitable for next-

generation sequencing (NGS) on the Oxford Nanopore platform, thus enabling real-time single-molecule

detection of biologically relevant RNA species.

Protocol (PDF)