Products

UDP-N-acetylglucosamine pyrophosphorylase (GlmU) Assays

UDP-N-acetylglucosamine pyrophosphorylase (GlmU) is a bifunctional enzyme that catalyzes transfer of

acetyl and uridyl groups onto glucosamine-1-P to generate UDP-GlcNAc, an essential precursor of

peptidoglycans. UDP-GlcNAc is involved in the synthesis of the N-acetylglucosamine polysaccharide

adhesin required for biofilm formation in bacteria.

The E. coli UDP-N-acetylglucosamine pyrophosphorylase (GlmU)Assay is based on measurement of the

pyrophosphate generated from the GlmU reaction.  The pyrophosphate is converted to phosphate by

pyrophosphatase and the phosphate is detected by light absorbance at 650 nm. The assay reactions and

detection can be performed by using 384-well or 96-well assay plates. Alternatively, the assay reaction can

be carried out in Eppendorf tubes and the signal is measured using a cuvette. The high throughput assay

can be used for screening inhibitors of E.coli GlmU in drug discovery research.  It may also be used for

characterization of E.coli GlmU.