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Optisol, Protein Solubility Screening Kit   - 재 판매 개시!!!

OptiSol™ Protein Solubility Kits help researchers identify formulations that protect target protein from

aggregation, or to solubilize an aggregated protein sample.

  

 

 

OptiSol Protein Solubilization Screening Kit contains a systematically varied array of buffers (from pH 3 to

pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that

enable the determination of conditions under which a particular protein sample is protected from aggregation

or can be de-aggregated. A total of 90 different formulations with solubility enhancerscan be tested in one

single, label-free experiment; the remaining 6 experiments are positive and negative control experiments.

The table below lists all reagents in an OptiSol Kit.

 

The OptiSol Protein Solubility Screening kit can be used for two fundamentally different purposes.

 

1. Protein Solubility Profiling (Protocol A)

 

Result: Optimal formulation (pH, salt, etc.) that maintains protein sample solubility under given stress

 

2. Solubilize an Aggregated Protein Sample (Protocol B)

 

Result: Optimal formulation (pH, salt, etc.) that converts aggregated protein into soluble form

 

For data analysis, use Protein Dashboard to assist in visualizing your data.

Click to download the Protein Dashboard

 

The Optisol Protein Solubility Screening kit is compatible with a wide diversity of protein sizes ranging from

several kDa to 250 kDa. Filter plates should be selected according to the expected MW of the solubilized

protein molecule.

 

FAQ

1. Will my aggregated protein sample ‘go in solution’ with the OptiSol reagents?

This depends on the nature of the protein aggregate. The only way to find out is by experimental testing –

and this is exactly what the OptiSol protein solubility screen does. Test 90 different mild formulations –

different pH, salts, polymers, sugars, polyols, detergent, reducing agents. If the protein sample is reversibly

aggregated, chances are the OptiSol screen will yield conditions that solubilize thesample.

The formulations employed are rather mild. The goal is to solubilize the aggregate and yield functional

protein. Will any aggregated protein samples get solubilized? No, some aggregates just contain ‘dead’

protein. You’ll need SDS or urea for such recalcitrant samples and obtain non-functional protein in return.

 

2. How much protein is required to run an OptiSol kit?

The protein concentration will be diluted by 11 times (15 µL/165 µL) in the reaction plate when the protein

solution is combined with the formulations. Thus, one has to start with a stock protein solution that is 11

times the final solution concentration in the reaction plate. About 2 mL of protein stock solution is adequate.

The required protein concentration depends on the extinction coefficientof the protein and the sensitivity of the

protein detection assay. In our laboratory we developed a UV280 method using half-width UV 96-well plate

and UV/vis plate reader. In our case, the UV280 absorbance in half-width UV280 wells has to be at least

around 0.4 (absolute absorbance units).

 

3. OptiPharma vs. OptiSol: Which one I should get?

All ingredients (buffers and additives) in an OptiPharma kit (except 3 control wells) are FDA approved

pharmaceutical ingredients. If you have pharmaceutical proteins in any stages of pharmaceutical formulation

development, OptiPharma is the one.

OptiSol is applicable to various types of protein work (e.g. pharmaceutical, industrial, food, plant,

crystallization work). Most additives in an OptiSol kit are not suitable for pharmaceutical formulations. For

example, if you have a crude protein mixture that you want to solubilize, OptiSol is a good choice to start

with.