종양 등의 biomolecules를 간단히 안정화하는 시약
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종양 등의 biomolecules를 간단히 안정화하는 시약, CellCover
현재 수술 후, 분리된 조직이 실온이나 PBS에 저장되거나 기타 다른 방법으로 보관되는데 이는 RNA/Protein expression pattern에 변화를 줄 수가 있습니다.
CellCover는 사람이나 동물의 종양조직, 백혈구세포, 배양세포(adherent, suspension, spheroids) 등의 DNA, RNA 및 protein을 one-step(시료량의 5배량 첨가로 완료) 으로 빠르게 안정화, 보존하는 시약입니다.
액체 질소를 사용하지 않더라도, CellCover에 노출된 세포들은 대사상태가 멈추게 되어 chemical degradation 없이,DNA, RNA 및 protein을 안정화시켜 시료를 몇 일 동안 보존 할 수 있습니다.
CellCover는 enzymatic hydrolysis를 shielding molecules로 안정화합니다. CellCover의 또 다른 장점은 핵산이나 단백질의 2차, 3차 구조가 유지되게 하기 때문에, CellCover를 이용한 조직이나 세포들은 in-vivo 상태와
같도록 DNA, RNA 및 protein을 유지시킨다는 것입니다. CellCover로 처리한 시료는 실온(25 ℃)에서 하루, 4 ℃에서 4 주까지 저장 할 수 있으며, 시료는 면역염색, flow cytometry, in situ hybridization 등에 적용 할 수
있습니다.
The Benefits
If treated with CellCover liquid freezing, cells grown on almost any substrate maintain in vivo morphology without chemical crosslinking of biomolecules. Compared to conventional formaldehyde fixation,
formalin sensitive epitopes are readily accessible for antibodies in immunohistochemical experiments. A unique and special feature of CellCover liquid freezing is that you can perform additional molecular
analysis on cells already analysed on morphological or molecular level: DNA, RNA (with high RINs) or protein can be isolated for subsequent applications after initial IHC, ICC or FACS analyses.
Even integrity of high molecular weight RNA is protected (e.g precurser rRNA, which are normally degradated after applying commonly used standards procedures) - and there is no need to hurry!
Time course: Cultured cells (SKMel-28) stored for 12 days in CellCover. RNA integrity was mesuared with Agilent Bioanalyzer. Results show a stable RIN(RNA integrity number) value of 10 from first till last day of the time course.
(Left) CellCover fixed cell on glass slide. Positive IHC signal for formalin-sensitive Epitope Vimentin.(Right) Formalin fixed cell on glass slide. No IHC signal for formalin-sensitive Epitope Vimentin.
(Left) Primary cells shows high cellular integrity by FACS analysis. (Right) Gated cells (R1) with red and green fluorescence signals.
The opportunities
These unique properties now enable new types of analyses of cellular functions. With CellCover liquid freezing, subpopulations of cells can be separated according to known properties by flow cytometry
and subsequently analysed for gene expression profiles on RNA or on protein-level. Thus, with CellCover liquid freezing it is easy to link transcriptome and proteome of a given cell population. In
comparison to commonly used molecular biological methods, CellCover adds following new analytical tools to bio-molecular research:
• Stabilize RNA and Protein simultaneously for several days, without doubt of molecule integrity
• Reliable analysis HMW-RNA and low stability enzymes even after day of storage
• Apply immunohistochemistry on formalin sensitive epitopes
• Apply in situ hybridization for analysis of gene expression
• Store cells conveniently for several days without change of protein or RNA expression profile or change in cells morphology as found with methanol
• Isolate subpopulations by flow cytometry based on protein labelling and perform subsequent transcriptome and/or proteome (MALDI) analyses.
Ordering informations
|
Catalog No. |
Product Name |
Size |
|
800-125 |
CellCover |
125 ml |
|
800-250 |
CellCover |
250 ml |
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