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Gel-based SARS-CoV-2 RdRp assay kit

The COVID-19 pandemic is caused by infection of SARS-CoV-2 or severe acute respiratory syndrome

coronavirus 2, a positive-sense single-stranded RNA virus. The RNA dependent RNA polymerase (RdRp) is

a key drug target for this deadly virus.

The gel-based assay can be used for visualization of the RNA molecules synthesized by the RNA

polymerase. It can also be used to visualize the enzyme inhibition by RdRp inhibitors. The gel based assay

result is consistent with the high throughput fluorescence-based assay.  Therefore it is useful for

confirmation of the inhibition from the high throughput screening. High throughput fluorescence-based

assayis also available for screening of RdRp inhibitors in a 384-well or 96-well pate format. Please find the

Protocol (PDF) of the gel-based RdRp assay.

The Gel-based SARS-CoV-2 RdRp assay kit (Catalog No. GS2RPA020KE) includes all the reagents for 20

samples in gel-based assays of SARS-CoV-2 RdRp activity.  It includes 75 µl of 10 x Buffer, 10 µl of 50 x

RNA template, 12 µl of 50 x NTPs (ATP, UTP, GTP, CTP), 10 µl of 50 x SARS-CoV-2 RdRp and 130 µl of 5 x

Gel loading buffer.

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