Products

The traditional method for nuclei isolation from animal cells/tissues depends on the use of non-ionic

detergent to lyse the plasma membrane and release nuclei which can be subsequently isolated by low-

speed centrifugation and further purified by density gradient. One major problem associated with the

traditional method is the clumping of nuclei after isolation, which, in some applications such as DNA and

RNA extraction, may not be a problem. However, in other applications such as single nucleussequencing or

proteomics, clumping of isolated nuclei represents a major problem. The anti-clumping nuclei storage buffer

is designed to address this issue. Nuclei resuspended in this buffer show significantly reduced clumping as

compared to those resuspended in PBS with 5% BSA. The nucleus suspension can be stored at 4℃ for days

without significant aggregation and change in morphology. The nuclei can also be frozen in the buffer at

-80℃ or in dry ice for transportation or long-term storage.

For spin column-based single nuclei isolation, refer to the list of available products.

NI-024 Minute™ Detergent-Free Single Nuclei Isolation Kit, 20 Preps

BN-020 Minute™ Single Nucleus Isolation Kit for Neuronal Tissues/Cells, 20 Preps

SN-047 Minute™ Single Nucleus Isolation Kit for Tissues/Cells, 20 Preps

AN-029 Single Nuclei and Cytosol Isolation Kit for Adipose Tissues/Cultured Adipocytes, 20 Preps

Comparison of the Storage Buffer with 5% BSA in PBS for Anti-Clumping Efficacy. Nuclei of frozen mouse

spleen and lung tissues were isolated by Nucleus Isolation Kit for Tissues/cells (Cat# SN-047, Invent

Biotechnologies, Plymouth MN). The isolated nuclei pellets were resuspended in 0.4 ml nuclei storage buffer

or 0.4 ml 5% BSA in PBS and incubated at 4℃ overnight.  The nuclei were resuspended by vortexing briefly

and stained with trypan blue to compare the nuclear morphologies.