Products

Cellular Fractionation, FOCUS™ Global Fractionation

FOCUS™ Global Fractionation kit is designed to fractionate complex biological samples into cytosolic and

membrane fractions. The resulting membrane fractions are subsequently fractionated into either peripheral

and integral membrane proteins or lipid raft associated proteins and detergent soluble membrane proteins.

Lipid rafts are membrane microdomains that are enriched in caveolin, cholesterol, glycolipids, sphingolipids

and glycosyl-phosphatidylinositol. Lipid rafts are also known as detergent-insoluble glycolipid-enriched

complexes (GEMs) or DIGs. Many signaling proteins, including glycosylphosphatidylinositol (GPI)-anchored

proteins, doubly-acylated tyrosine kinases of the Src family, and transmembrane proteins, are located in

lipid rafts. Lipid raft localized proteins have been shown to be involvedin intracellular membrane trafficking

and signaling.

FOCUS™ Global Fractionation is designed for >50 preps, where one prep is:

• 100mg mammalian tissue

• 50µl wet animal cell pellet

• 50µl wet yeast pellet

• 50µl wet bacteria pellet

• 250mg plant tissue

Features;

• Fractionate integral, peripheral and lipid raft associated proteins.

• Fractionation of complex proteomes into multiple, specific fractions.

Application;

• Selective fractionation of membrane proteins from tissues, cells, plants, yeast, bacteria, insects, and other

  sources.

• Fractionation of membrane proteins from lipid rafts.

• Suitable for membrane protein preparation for 2D gel electrophoresis and other applications.

• Suitable for proteomics and cell signaling studies.

 Instruction sheet (PDF)