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Ornithine ELISA kit

Free L-Arginine (Arg) is provided by diet, de novo synthesis and protein turnover. De novo synthesis is sufficiently important to not consider Arg as an essential amino acid. However, in some circumstances – eg. early development, infection and inflammation – de novo synthesis is not enough and require dietary Arg, which is thus classified as a conditionally-essential amino acid. Whatever the source, Arg can be metabolized through different degrading pathways which include i) the nitric oxide synthase (NOS) pathway providing Citrulline and NO, as well as ii) the well-known Arginase pathway leading to the production of Urea and L-Ornithine (Orn). 

Cross-validation of L-Ornithine ELISA and LC/MS data in human plasma samples Orn was quantified in human plasma samples from healthy subjects using IS-I-1000R ELISA kit or by LC/MS. Correlation study showed R2=0.9636, thereby confirming the accuracy of the immunoassay

Specifications

Typical standard curve of Ornithine ELISA Typical standard curve obtained with the Orn ELISA kit. In this competitive enzyme immunoassay, optical density is inversely correlated with Orn levels within a linear range of 20 - 781.25µM. (example data - do not use for calculation).

Instruction sheet (PDF)

References;

1. Caldwell et al., Arginase: A Multifaceted Enzyme Important in Health and Disease, Physiol Rev., 2018 

2. Niu et al., Arginase: An emerging and promising therapeutic target for cancer treatment, Biomed Pharmacother. 2022  

3. Apiz-Saab et al.,IPancreatic tumors activate arginine biosynthesis to adapt to myeloid-driven amino acid stress, bioRxiv 2022