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Universal Reference Total Protein

Universal Reference Total Protein

Zyagen Universal Reference Total Protein is a high quality standard for protein expression analysis prepared at industrial

scales to minimize lot-to-lot variation. All tissues (organs) of several male and female C57BL/6 mice are freshly dissected,

cut in very small pieces, snap frozen, grinding to powder, and homogenized in protein lysis buffer supplemented with a

cocktail of 7 protease inhibitors to minimize proteolysis.

The quality of protein as indicated by the absence of smear (no degradation) and sharpness and resolution of the protein

bands are verified by denatured SDS-PAGE with Coomassie blue staining. The integrity of protein is tested by

immunoblotting using specific beta-actin antibody.

The extracted protein is provided in 1.5 ml-tubes at a concentration of 5mg/ml and ready for immediate use in Western

blotting, immunoprecipitation, SDS-PAGE, isoelectric focusing gels and SDS-capillary electrophoresis. Protein can also be

provided in denatured SDS Sample buffer for only Western blotting use. Protein is stable for one year if stored properly

at -70^oC.

Features:

- High quality intact total protein prepared at industrial scales to minimize lot-to-lot variation.

- Consistent control for standard data set comparisons

- Extracted from all rat tissues for broad gene coverage.

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Ordering informations

Mouse Universal Reference Total Protein

Catalog No.	Product Name	Size
MT-UR-100	Mouse Universal Reference Total Protein	1 x 0.1mg
MT-UR-500	Mouse Universal Reference Total Protein	2 x 0.5mg
MT-UR-1000	Mouse Universal Reference Total Protein	2 x 1mg

Rat Universal Reference Total Protein

Catalog No.	Product Name	Size
RT-UR-100	Rat Universal Reference Total Protein	1 x 0.1mg
RT-UR-500	Rat Universal Reference Total Protein	2 x 0.5mg
RT-UR-1000	Rat Universal Reference Total Protein	2 x 1mg

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