Minute™ Total protein extraction kit for animal cultured cells and tis…
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Minute™ Total protein extraction kit for animal cultured cells and tissues
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Total Protein Extraction Kit에는 kit에 denaturing cell lysis buffer와 native cell lysis buffer가 포함되어 있어, 용도에 따라 2가지의 단백질을 얻을 수가 있으며, detergent-free kits의 Cell lysis buffer에는
계면활성제 및 EDTA가 들어 있지 않습니다.
Very simple steps
Invent Biotechnologies' Minute™ total protein extraction kit for animal cultured cells and tissues is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml
collection tubes. The kit is designed to rapidly extract total proteins from animal cells and tissues (invertebrate and vertebrate) for protein analysis and further purification. Since protein profiles
extracted by denaturing and native cell lysis buffer are not identical, for a given application one might be superior to the other. This kit provides both denaturing and native cell lysis buffers for
users to test and select the best one for a specific application. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 µl and as large as 500 µl.
This unique feature is very useful in situations where available starting material is a limiting factor. Total proteins can be extracted from cultured cells/tissues in 1-8 min with high yield (2-8 mg/ml).
Extracted proteins can be use as a good starting material for protein analysis and small scale protein purification in column chromatography.
Minute™ total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells and tissues for applications such as SDS-PAGE, immunoblottings,
IP, ELISA, enzyme assays and other applications. This kit provides the most rapid method currently available for preparation of whole cell protein extract. Extracted proteins can also be use as
a good starting material for small scale protein purification in column chromatography.
Protein profiles of animal tissues extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
Lane 1, drosophila larvae; lane 2, drosophila pupa; 3, adult drosophila; 4, gold fish muscle and 5, mouse liver
Ordering information
Catalog No. | Product Name | Kit components | Amount |
SD-001/SN-002 | Minute™ Total protein extraction kit | Denaturing cell lysis buffer(SD-001-L) | 25 ml |
Native cell lysis buffer(SN-002-L) | 25 ml | ||
Protein extraction filter cartridges(P-001) | 50 | ||
Collection tubes with cap(P-002) | 50 | ||
Plastic rods(P-003) | 2 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
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