Minute™ Detergent-free protein extraction kit for animal cultured cell…
본문
Minute™ Detergent-free protein extraction kit for animal cultured cells and tissues
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Total Protein Extraction Kit에는 kit에 denaturing cell lysis buffer와 native cell lysis buffer가 포함되어 있어, 용도에 따라 2가지의 단백질을 얻을 수가 있으며, detergent-free kits의 Cell lysis buffer에는
detergent 및 EDTA가 들어 있지 않습니다.
Very simple steps
Invent Biotechnologies' Minute™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is
designed to rapidly extract detergent-free total proteins from cultured cells (insect /mammalian/other cultured cells) and animal tissues (invertebrate and vertebrate). The protein extraction buffers
do not contain any detergent and EDTA. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 μl and as large as 500 μl. This unique feature is very
useful in situations where available starting material is a limiting factor. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 min with high yield (1-5 mg/ml).
Minute™ detergent-free total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells/tussues for applications such as proteomics (LC/MS),
IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high concentration of whole cell extract.
Because of high yield of protein extract and low salt concentration, the protein extract can be used directly for downstream applications without desalting and protein concentration.
Figure 1. Comparison of protein profiles of different animal tissues extracted by denaturing lysis buffer and detergent-free extraction buffer.
Lane 1, Drosophila cultured cells (SL2); Lane 2, Drosophila adult flies; Lane 3, gold fish muscle; Lane 4, mouse liver. Extracted proteins were separated in 10% SDS-PAGE and stained with
coomassie blue.
Ordering information
Catalog No. | Product Name | Kit components | Amount |
SN-006 | Minute™ Detergent-free total protein extraction kit | Buffer A(SN-006-A) | 15 ml |
Buffer A(SN-006-A) | 15 ml | ||
Protein extraction filter cartridges(P-001) | 50 | ||
Collection Tubes with Cap(P-002) | 50 | ||
Plastic rods(P-003) | 4 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
댓글목록
등록된 댓글이 없습니다.