Minute™ Total protein extraction kit for plant tissues
본문
Minute™ Total protein extraction kit for plant tissues
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Total Protein Extraction Kit에는 kit에 denaturing cell lysis buffer와 native cell lysis buffer가 포함되어 있어, 용도에 따라 2가지의 단백질을 얻을 수가 있으며, detergent-free kits의 Cell lysis buffer에는
detergent 및 EDTA가 들어 있지 않습니다.
Very simple steps
Invent Biotechnologies Minute™ total protein extraction kit for plant tissues is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes.
The kit is designed to rapidly extract denatured or native proteins from plant tissues (leaves, seeds and soft stem and roots etc.). Since protein profiles extracted by denaturing and native cell lysis
buffer are not identical, for a given application one might be superior to the other. This kit provides both denaturing and native cell lysis buffers for users to test and select the best one for a
specific application. Due to the use of the protein extraction filter cartridges, total plant soluble proteins can be extracted from 20-200 mg plant tissue in less than 8 min with high protein yield
(2-8 mg/ml). Extracted proteins can be use as a good starting material for protein analysis and small scale protein purification in column chromatography.
Minute™ total protein extraction kit for plant tissues is designed to rapidly extract total proteins from fresh or frozen plant tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP,
enzyme assays and other applications. This kit provides a very rapid method for preparation of soluble protein extract from plant tissues. Extracted proteins can be used as a good starting material
for small scale protein purification in column chromatography.
Figure 1. Plant protein profiles extracted by denaturing lysis buffer and native lysis buffer. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
Lane 1, snow pea; lane 2, corn kernel; 3, broccoli leaves; 4, spinach leaves and 5, chili pepper leaves.
Ordering information
Catalog No. | Product Name | Kit components | Amount |
SD-008/SN-009 | Minute™ total protein extraction kit for plant tissues | Denaturing lysis buffer | 25 ml |
Native lysis buffer | 25 ml | ||
Protein extraction filter cartridges(P-001) | 50 | ||
Collection Tubes with Cap(P-002) | 50 | ||
Plastic rods(P-003) | 4 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
댓글목록
등록된 댓글이 없습니다.