Minute™ Detergent-free protein extraction kit for plant tissues
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Minute™ Detergent-free protein extraction kit for plant tissues
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Total Protein Extraction Kit에는 kit에 denaturing cell lysisbuffer와 native cell lysis buffer가 포함되어 있어, 용도에 따라 2가지의 단백질을 얻을 수가 있으며, detergent-free kits의 Cell lysis buffer에는
detergent 및 EDTA가 들어 있지 않습니다.
Very simple steps
Invent Biotechnologies' Minute™ detergent-free plant protein extraction kit is composed of optimized protein extraction buffer and protein extraction filter cartridges with 2.0 ml collection tubes.
The kit is designed to rapidly extract detergent-free total proteins from plant tissues. The protein extraction buffer does not contain any detergent and organic solvent. Due to the use of the protein
extraction filter cartridges, the extraction volume can be as small as 50 μl and as large as 500 μl. Detergent-free total proteins can be extracted from very small plant samples (leaves, seeds, soft
stems etc.) in less than 8 min with high yield (1-6 mg/ml). Minute™ detergent-free plant protein extraction kit is designed to rapidly extract water soluble proteins from plant tissues for applications
such as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high
concentration of protein extract.
Figure 1. Comparison of plant protein profiles extracted by denaturing lysis buffer and detergent-free extraction buffer.
Lane 1, Corn kernel; Lane 2, Broccoli leaves; Lane 3, Spinach leaves. Extracted proteins were separated in 10% SDS-PAGE and stained with coomassie blue.
Ordering information
Catalog No. | Product Name | Kit components | Amount |
SN-010 | Minute™ Detergent-fee protein extraction kit for plant tissues | DF-llysis buffer(with 5 beads)(SN-010-L) | 25 ml |
Protein extraction filter cartridges(P-001) | 50 | ||
Collection Tubes with Cap(P-002) | 50 | ||
Plastic rods(P-003) | 4 |
* For 50 preps
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