Minute™ Plasma Membrane Protein Isolation Kit
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Minute™ Plasma Membrane Protein Isolation Kit
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다.
Very simple steps
Invent Biotechnologies Minute™ plasma membrane protein isolation kit is composed of optimized buffers (detergent and EDTA free) and protein extraction filter cartridges with 2.0 ml
collection tubes. The kit is designed to rapidly isolate native total membrane proteins (organelle membrane proteins) and native plasma membrane proteins from cultured mammalian
cells or tissues. Due to the use of protein extraction filter cartridges, the membrane protein isolation is simple, easy and user friendly with high yield. Unlike many commercial membrane
preparation kits that require large amount of starting cells (5 millions and up). This kit offers wide range of starting cells (1-50 millions/sample). The buffers are detergent and EDTA
free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less than 45 min.
Figure 1
A. SDS-PAGE profiles of total cell lysate (TC) and isolated plasma membrane proteins (PM) from human and rat cultured cells. Lane 1, Human lung cancer cell A549 total cell
lysate; Lane 2, Isolated PM of A549; Lane 3, PM of rat REL-6TN cells; Lane 4, Total cell lysate of rat REL-6TN cells.
B. Western blottings : Proteins in A were transferred to nitrocellulose membrane and probed with anti-Na/K ATPase alpha1, a plasma membrane marker (Upstate, Clone 464.6))
and anti-lamin B1, a nuclear envelope marker (ab16048, abcam Cambridge, MA). The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD).
C. Densitometry measurement of Na/K ATPase alpha1 signals in B (TC vs. PM). Total cell lysates were extracted by Minute Denaturing Total Protein Extraction Kit (SD-001, I
nvent Biotechnologies, Eden Prairie, MN).
Figure 2
A. SDS-PAGE profiles of isolated total membrane proteins from mouse tissues (T=Total Cell lysate, C=Cytosol Fraction, M= Total Membrane Fraction)
B. Proteins shown in A were transferred to a nitrocellulose membrane and probed with rabbit-anti mouse pan-cadherin (ab6529,abcam, Cambridge, MA), and anti-actin by Western
blotting. The specific protein bands were visualized by a color metric substrate Opti-4CN (Bio-RAD). Signals of pan-cadherin (a 130 kda plasma membrane marker) were
significantly enhanced in total membrane protein fractions. Total cell lysates were extracted by MinuteTM Denaturing Total Protein Extraction Kit (SD-001, Invent Biotechnologies,
Eden Prairie, MN).
Figure 3
A. SDS-PAGE profiles of total membrane protein fraction (TM) and isolated plasma membrane protein fraction from mouse tissues.
B. Western blotting of proteins in A were transferred to nitrocellulose membrane and probed with rabbit anti-cadherin (abcam, Cambridge, MA). The specific protein bands were
visualized by a color metric substrate Opti-4CN (Bio-RAD).
C. Densitometry measurement of cadherin signals in B (TM vs. PM).
Ordering information
Catalog No. | Product Name | Kit components | Amount |
SM-005 | Minute™ Plasma Membrane Protein Isolation Kit | Buffer A (SM-005-A) | 25 ml |
Buffer B (SM-005-B) | 10 ml | ||
Protein extraction filter cartridges(P-001) | 50 | ||
Collection tubes with cap(P-002) | 50 | ||
Plastic rods (P-003) | 4 | ||
Tissue dissociation beads (P-004) | 10 g |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
- 이전글Minute™ Mitochondria Membrane Protein Isolation Kit 13.12.16
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