Products

Minute™ Mitochondria Membrane Protein Isolation Kit

본문

Minute™ Mitochondria Membrane Protein Isolation Kit 
Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약을 조합하여, 빠르고 쉽게, high yield로 단백질 등을 추출 할 수 있는 kit 입니다.
  
 클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요

Invent Biotechnologies사의 독자적 기술인 스핀 컬럼과 시약으로, 다양한 용량의 시료에서 간편, 신속하면서도 high yield로 단백질 등을 추출 할 수 있으며, 시료의 sonication 처리는 필요하지 
않습니다. 본 제품을 사용하여 추출, 정제한 단백질은 SDS-PAGE, immunoblotting, ELISA, immunoprecipitation, protein localization, gel mobility shift assays 등의 연구에 사용할 수 있습니다. 
 
Very simple steps 
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요
Invent Biotechnologies' Minute™ mitochondria membrane protein isolation kit is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. 
The kit is designed to rapidly isolate native mitochondria proteins from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation 
procedure is simple, easy and user friendly with high yield. Unlike many commercial mitochondria preparation kits, this kit offers wide range of starting cells (5-50 million/sample) 
and intact mitochondria are isolated. The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less
than 30 min.
클릭하시면 닫힙니다.이미지 저장을 원하시면 마우스 오른쪽클릭후 '다른이름으로 저장'을 하세요 
Figure 1. Enrichment of Mitochondrial Marker (Ubiquinol-Cytochrome C Reductase Core Protein) with Minute Mitochondria Isolation Kit.
 
A. SDS-PAGE profiles of total protein extract vs. isolated mitochondrial proteins. Lanes 1, 3 5, total proteins from isolated mouse kidney cells, mouse skeletal muscle and liver 
   tissues respectively. Lanes 2, 4, 6, isolated mitochondrial proteins from mouse kidney cells, mouse skeletal muscle and liver tissues respectively.
B. Western blotting. Proteins in A were transferred to nitrocellulose membranes and probed with anti-ubiquinol-   cytochrome C reductase core protein (ab96333, abcam, 
   Cambridge, MA) and anti-lamin B1 (ab16048,a nuclear envelope marker protein. abcam, Cambridge, MA).
C. Densitometry measurement of mitochondrial marker signals.
  
 
Ordering information 
Catalog No.
Product Name
Kit components
Amount
MP-007
 Minute™ Mitochondria Membrane
 Protein Isolation Kit
 Buffer A (SN-007-A)
30 ml
 Buffer B (SN-007-B)
15 ml
 Protein extraction filter cartridges(P-001)
50
 Collection tubes with cap(P-002)
50
 Plastic rods (P-003)
4
 Tissue dissociation beads (P-004)
10 g
* For 50 preps 
 
▣ 관련 페이지 ; Invent Biotechologies, Inc.


댓글목록

등록된 댓글이 없습니다.

Copyright by Sambo Medical Co. All rights reserved.