Products

Plasmid DNA Extraction Strip Cards

Plasmid DNA Extraction Strip Card (12 x 8 sample strips)

Description

LyseNow^? Plasmid DNA Extraction Strip Cards features proprietary lysis buffer impregnated filter paper discs that

are assembled between two layers of transparent PET sheets and spaced out to align with one row of a 96 well

plate format. The filter paper discs lyse E. coli, or other host cells on contact, release plasmid DNA; transparent

card allows for the easy alignment of the discs over one row of a 96 well plate,the discs can be effortlessly

pushed down to 96 well pl`ate wells with sterile pipette tips; nucleic acids from the host cells, especially plasmid

DNA because of their smaller size, can then be easily eluted off with 10 minutes shaking.

Elution can be used directly for PCR or sequencing. In one instance, more than 700 bp high quality sequencing

data were generated with CE sequencing from lysates of overnight culture harboring a 14 kb low copy number

plasmid. LyseNow^? Plasmid DNA Extraction Strip Card produces little plastic waste and no any chemical waste

at all. Up to 96 colonies can be processed for PCR ready DNA extraction in about 30 minutes. 

 Kit contents

Instruction sheet  (PDF)

Plasmid DNA Extraction Card (96 Card)

Description

LyseNow^? Plasmid DNA Extraction Cards feature proprietary lysis buffer impregnated filter paper discs that are

assembled between two layers of transparent PET sheets and arranged in a 96 well plate format. The filter paper

discs lyse E. coli, or other host cells on contact, release plasmid DNA; transparent card allows for the easy

alignment of the discs over a 96 well plate, the discs can be effortlessly pusheddown to 96 well plate wells with

sterile pipette tips; nucleic acids from the host cells, especially recombinant DNA because of their smaller size, can

then be easily eluted off with 10 minutes shaking. Elution can be used directly for

PCR or sequencing. In one instance, more than 700 bp good sequencing data were generated with CE sequencing

from lysates of overnight culture harboring a 14 kb low copy number plasmid. LyseNow^? Plasmid DNA Extraction

Card produces little plastic waste and no any chemical waste at all.

Kit contents

Instruction sheet  (PDF)

Catalog No.

Product Name

Size

E500-8

Plasmid DNA Extraction Strip Card

12 x 8 sample strips

E500

Plasmid DNA Extraction Card

96 Card