Products

Product name

Anti-mouse IgG F(ab)-Atto488

Target species

Mouse

Description

Atto-labeled goat-anti mouse IgG F(ab) fragment belong to the new generation of

fluorescent antibodies characterized by their exceptional fluorescence intensity and

superior photostability. Goat anti-mouse IgG F(ab)-Atto488 is specifically suited for

high and super resolution microscopy, due to the high photostability and strong

fluorescence quantum yield.

Conjugates of F(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access;

- prevention of interactions with Fc receptor-bearing membranes due to the absence

  of the Fc region in Fab fragments;

Specifications

Anti-mouse IgG F(ab) Atto488 (H+L), Immunofluorescence: 1:300-1:500, Degree of

labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence.

For further information please see DataSheet.

Optical properties

 λex 501nm, λem 523nm

Form

Lyophilized

Concentration

2mg/ml

Content

0.25mg (2mg/ml) goat-anti mouse IgG (Fab) Atto488*

1.0ml Glycerol buffer

Product documentation

Buffer

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride,

5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

 Column affinity purified, IEX chromatography after papain cleavage.

Storage condition

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping condition

Shipped at ambient temperature

Product name

Anti-mouse IgG F(ab)-Atto565

Target species

Mouse

Description

Atto-labeled secondary antibodies F(ab) fragments belong to the new generation of

fluorescent antibodies, characterized by their exceptional fluorescence intensity and

superior photostability. Goat anti-mouse IgG (Fab) Atto565 is specifically suited for

high resolution microscopy, due to the high photostability and strong fluorescence

quantum yield.

Conjugates of F(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access

- prevention of interactions with Fc receptor-bearing membranes due to the

   absence of the Fc region in Fab fragments.

Specifications

Anti-mouse igG F(ab)-Atto565 (H+L), Immunofluorescence: 1:300-1:500, Degree of

labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence.

For further information please see DataSheet.

Optical properties

 λex 563nm, λem 592nm

Form

Lyophilized

Concentration

2mg/ml

Content

0.25mg (2mg/ml) goat-anti mouse IgG (Fab) Atto565*

1.0ml Glycerol buffer

Product documentation

Buffer

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride,

5 mM sodium azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

 Column affinity purified, IEX chromatography after papain cleavage.

Storage condition

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping condition

Shipped at ambient temperature

Product name

Anti-Mouse IgG  F(ab)-Atto594

Target species

Mouse

Description

Atto-labeled secondary antibodies F(ab) fragments belong to the new generation of

fluorescent antibodies, characterized by their exceptional fluorescence intensity and

superior photostability. Goat anti-mouse IgG (Fab) Atto594 is specifically suited for

high resolution microscopy, due to the high photostability and strong fluorescence

quantum yield.

Conjugates of F(ab) fragments are preferable to whole antibody conjugates:

- for cellular structures which are sterically difficult to access

- prevention of interactions with Fc receptor-bearing membranes due to the absence of

   the Fc region in Fab fragments.

Specifications

Goat anti-mouse IgG F(ab) Atto594 (H+L), Immunofluorescence: 1:300-1:500, Degree

of labeling (DOL) : >1-3, Unconjugated dye ≤5% of total fluorescence.

For further information please see DataSheet.

Optical properties

 λex 594nm, λem 627nm

Form

Lyophilized

Concentration

2mg/ml

Content

0.25mg (2mg/ml) goat-anti mouse IgG (Fab) Atto565*

1.0ml Glycerol buffer
Product documentation

Buffer

50% glycerol, 0.01 M sodium phosphate, 0.1 M sodium chloride, 5 mM sodium
azide, pH 7.4.

Clonality

Polyclonal

Isotype

IgG

Purification notes

 Column affinity purified, IEX chromatography after papain cleavage.

Storage condition

Store as glycerol stock at -20°C. Avoid repeated freeze / thaw cycles.

Shipping condition

Shipped at ambient temperature