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Fast Yeast Transformation Kit™

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Fast Yeast Transformation Kit™

빠르고 간단하게 one-step으로,  효모 competent cell를 조제 할 수 있는 kit입니다.

 

Fast Yeast Transformation™ kit is a rapid single step yeast transformation kit that takes less than 10 minutes

to prepare competent yeast cells. The competent yeast cells can be used immediately or frozen for later

use. This method is suitable for both circular and linear plasmid transformations. The protocol involves

simply suspending yeast cells in the supplied Competent Buffer and the cells are then ready to receive

transforming DNA. Introduce DNA to the competent cells and incubate for transformation.

 

Features

High transformation efficiency: 105-106 transformants/μg circular DNA Broad spectrum: Compatible with C. albicans, S. pombe, P. pastoris, or S. cerevisiae Transformation procedure takes less than an hour Frozen competent cells are good for use for up to 6 months Simple protocol for multiple plasmid transformation

 

Applications

For the generation of competent yeast cells Generates plasmids suitable for multiple downstream applications

 

References

Takada, Y. et al (2014) FEMS Yeast Res. 14:261 Zeng, G. et al (2014) Nature Protocols. 9:464 Jaiswal, D.K. et al (2014) Scientific Reports. doi:10.1038/srep04177 Liu, J. et al (2013) Appl. Microbiol. Biot. 97:6467 Liu, J. et al (2013) J. Biotech. 168:446 Zeng, G. et al (2012) Mol. Biol. Cell. 23:3485 Kanatsu, Y. et al (2012) J. Biochem. 152:63 Ide, M. et al (2012) Arch. Microbiol. 194:243 Kim, J. et al (2011) Plant Cell Physiol. 52:2136 Ito, J. et al (2011) Plant Cell Physiol. 52:539 Yang, J. et al (2011) Mol. Microbiol. 79: 872 Nakamura, Y. et al (2011) Anal. Biochem. 412:159 Takano, S. et al (2010) Plant Cell Physiol. 51:62 Koszewski, N.J. et al (2010) J. Ster. Biochem. Mol. Biol. 120:200 Kikis, E. A. et al (2009) PLoS Genet. 5:e1000352 Aihara, T. et al (2009) Biol. Reprod. 80:762 Matsuda, M. et al (2009) Microb. Infect. 11:646 Hayashi, C. et al (2008) J. Biol. Chem. 283:14801 Tai, Y.S. (2008) Mol. Biol. Rep. 35:337 Lin, Y. F. et al (2007) J. Biol. Chem. 282:16783 Morokuma, Y. et al (2007) J. Biol. Chem. 282:24806 Tai, Y. (2007) Am. J. Plant Physiol. 295 Ono, Y. et al (2006) J. Biol. Chem. 281:18519 Lin, Y. et al (2006) PNAS. 103:15617 Xia, H. et al (2003) Plant Cell. 15:449 Ueki, N. and Hayman, M. J. (2003) J. Biol. Chem. 278:24858 Asawatreratanakul, K. et al (2003) Euro. J. Biochem. 270:4671 Uhl, M. et al (2003) EMBO J. 22:2668 Murooka, Y. et al (2002) J. Biosci. Bioeng. 94:225 Osman, A. et al (2001) J. Biol. Chem. 276:10072


Ordering informations

Catalog No.

Product Name

Size

GZ-1

Fast Yeast Transformation™

120 Preps


▣ 관련 페이지 ; G-Biosciences


 

 

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