Repro-miRNA
본문
Repro-miRNA
Technical information
a. Purity :
90-95% by HPLC
b. Sequence validation
by mass spec
by mass spec
c. Usage
- Disslove into RNAase-free water to make 20 µM stock solution, aliquote and store at -80°C for longer shelf life (up to 12 months), avoid repeated freeze/thaw cycles.
- Optimize the transfection condition using Cell Death control siRNA in 24 well. Parameters needed to be optimized: type of transfection reagent, amount of transfection reagent, and amount of siRNA. The effect of Cell Death siRNA can be observed 48-72 hours post transfection under microscope.
- Use the optimized conditions for miRNA experiment. Repeated transfection is needed for sustained effects over a long period of time.
Biological Activity
It is well accepted that microRNAs (miRNAs) play a key role in stem cell self-renewal, differentiation, and
reprogramming 1. More recent studies have demonstrated that specific miRNAs can significantly promote
reprogramming of somatic cells toward pluripotency, or substitute reprogramming transcription factors.
Furthermore, it was reported that direct transfection of synthetic mature miRNAs (a combination of miR200c/
miR302s/miR369s families) alone 2 allowed generation of mouse and human iPSCs without any genetic
modifications. Those studies opened up the possibilities of using these synthetic mature miRNAs to enable or
facilitate reprogramming experiments in different studies.
Reference
- Sridharan and Plath, Small RNAs loom large during reprogramming. Cell Stem Cell 8, 599-601, (2011).
- Miyoshi et al. Reprogramming of mouse and human cells to pluripotency using mature microRNAs. Cell Stem Cell 8, 633-638, (2011).
- Anokye-Danso et al. Highly efficient miRNA-mediated reprogramming of mouse and human somatic cells to pluripotency. Cell Stem Cell 8, 376-388, (2011).
- Subramanyam et al. Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. Nat Biotechnol. 29, 443-448, (2011).
- Melton et al. Opposing microRNA families regulate self-renewal in mouse embryonic stem cells. Nature 463, 621-626, (2010).
- Judson et al. Embryonic stem cell-specific microRNAs promote induced pluripotency. Nat Biotechnol. 27, 459-461, (2009).
Ordering information ;
- K6471-5 1 unit each of following products
- N2003-5 hsa-miR200c-3p mimics (5 nmol)
- N3021-5 hsa-miR302a mimics (5 nmol)
- N3022-5 hsa-miR302b mimics (5 nmol)
- N3023-5 hsa-miR302c mimics (5 nmol)
- N3024-5 hsa-miR302d mimics (5 nmol)
- N3693-5 hsa-miR369-3p mimics (5 nmol)
- N3695-5 hsa-miR369-5p mimics (5 nmol)
- N0000-1 miR-negative control (1 nmol)
- N1111-5 Cell Death transfection efficiency siRNA control (5 nmol)
* Note : 5 nmol miRNA is enough for 400 transfection in 6-well format under normal condition
댓글목록
등록된 댓글이 없습니다.