Hemoglobin Depletion From Erythrocytes
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HemoVoid™ - Hemoglobin Depletion Reagent Kit
Features
- Hemoglobin voids in flow-through >98%, with <30 minute bind/wash/elute protocol
- Hemoglobin removal from whole blood, hemolyzed serum and red cell lysates for proteomics study
- Enrichment of hemoglobin variants.
- Low abundance protein and enzyme enrichment
- Disposable, cost-effective
- Mild elution maintains tertiary structure and simple transfer to secondary analysis
- Removes hemoglobin from species including human, sheep, bovine, goat, etc.
- The eluted fractions retain their enzymatic and biological activity
HemoVoid™ , a silica-based protein enrichment matrix, removes hemoglobin from erythrocyte lysate samples while concentrating low abundance, and/or low molecular weight proteins. The HemoVoid™ protocol uses mild buffers; the protocol conditions are so gentle that native enzyme activity is retained in elution fractions. HemoVoid™ derives from a silica-based library of
individual mixed-mode ligand combinations (ionic, hydrophobic, aromatic, polymer). The library was designed to facilitate weak binding of proteins, allowing for rapid elution from the matrix
without any foreknowledge of the variety of proteins contained in the starting sample. HemoVoid™ depletes hemoglobin from red cell lysates while improving the resolution of less abundant
blood proteins.
Reference
Mizukawa, B., George, A., Pushkaran, S., Weckbach, L., Kalinyak, K., Heubi, J. E. and Kalfa, T. A. , Cooperating G6PD mutations associated with severe neonatal hyperbilirubinemia and cholestasis. Pediatric
Blood & Cancer.
Functional 20S proteasomes in mature human red blood cellsSudha Neelam1, David G Kakhniashvili1, Stephan Wilkens1, Stephen D Levene2 and Steven R Goodman1Exp. Biol. Med. 2011;236:580-591
doi:10.1258/ebm.2011.010394
Ordering information ;
1. 10 Preps
2. 50 Preps
3. 100 Preps
* 300µL of samples can be processed per prep.
- 이전글ProCipitate, substitute to phenol / chloroform 12.08.18
- 다음글BindPro Metabolomics Kit 12.05.07
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