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                  Very simple steps

Protein extraction from cell wall-containing microbes is a frequent procedure in bio-research Labs. The 

methods for protein extraction from these microbes are usually harsh, tedious and not sufficiently reliable.

YT-015 provides an instrument-free, rapid and gentle way for extracting proteins from microbes with thick

and strong cell walls. These microbes include but not limited to yeast, filamentous fungus, grampositive and

negative bacteria, insect eggs and microalgae. This kit contains optimized denaturing and native protein

extraction buffers for user to choose from. Unlike many other methods of which harsh conditions, such as

8 M urea, glass bead lysis using an instrument, and boiling the sample with alkaline extraction etc., are

used for yeast protein extraction. Proteins in certain molecular weight range are usually preferentially

extracted with these methods. This kit features a single tube protocol, full rangeof yeast proteins are

extracted without bias. The kit works well for both log phase and stationary phase microbes. The whole

procedure takes less than 10 min to complete and the protein yield is in the range of 2-5 mg/ml. The

materials provided are sufficient for 50 extractions.