Minute™ Yeast Mitochondria Enrichment Kit
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| Minute™ Yeast Mitochondria Enrichment Kit
Traditional protocols for yeast mitochondria isolation/extraction involve a range of centrifugation-based subcellular fractionation procedures. Typically the techniques include spheroplast preparation, glass-bead lysis using a homogenization instrument, differential centrifugation and several density gradient procedures using a variety of gradient media with ultracentrifugation. The procedures are very tedious and time consuming. Here we feature a simple and rapid protocol for yeast mitochondriaenrichment. The procedure is gentle and instrument-free. Nativ mitochondrial proteins can be isolated from yeast in about one hour without ultracentrifugation. This kit contains optimized detergent-free protein extraction buffers. The protein yield is in the range of 150-250 μg/sample. The materials provided are sufficient for 50 extractions. |
Instruction sheet (PDF)
Ordering information
| Catalog No. | Product Name | Kit components | Amount |
| YM-017 | Minute™ Yeast Mitochondria Enrichment Kit | Buffer A | 30 ml |
| Buffer B | 10 ml | ||
| Protein Extraction Powder | 5g | ||
| 1.5 ml Microfuge Tube | 50 | ||
| Pestles | 4 |
* For 50 preps
▣ 관련 페이지 ; Invent Biotechologies, Inc.
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