Products

Atto-Actin(alpha cardiac actin, bovine cardiac muscle)

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Atto-Actin (alpha cardiac actin, bovine cardiac muscle)

 

The Colours of Actin!

The bright way to visualize actin interactions.

 

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Atto-Actins are important tools for investigating actin dynamics.

 

Ready-to-use fluorescent actin conjugates suited for numerous state-of-the-art techniques. Atto-actins are

used for all types of fluorescence microscopes: from epifluorescence to high resolution laser microscopy,

biophysical spectroscipcal methods and biochemical applications. The novel Atto-Actins are characterized

by high photo stabilty, strong absorption and high fluorescence quantum yields - some of several features

demanded for todays imaging techniques.    

 

These fluorescent actin conjugates are prepared by esterification of lysine residues with activated Atto dyes.

Atto-Actins are validated to possess the polymerization properties of native actin.

 

Atto-Actins are worldwide exclusively manufactured by Hypermol.

 

Applications

TIRF Microscopy, FRET, Confocal Microscopy, Epifluorescence Microscopy, Biophysical Spectroscopical

Techniques, Biochemical Actin Interaction Assays.

 

 

Atto390-Actin (alpha-cardiac actin, bovine)

 

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ATTO 390

 

High fluorescence yield

• Large Stokes-shift

• Wel suited for all types of fluorescence microscopes

 

 

Description

Protein

 

Atto390-Actin

Origin

 

Alpha cardiac actin, rabbit

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from bovine cardiacl muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto390-Actin is chemically modified

G-actin with an Atto390 fluorescent group covalently bound to lysine residues.

Atto390-Actin (λem 390nm, λem 479nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

 

Buffer

 

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from bovine cardiac muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-actin is kept on ice

and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Atto532-Actin (alpha-cardiac actin, bovine)

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ATTO 532

 

High fluorescence yield

• High photostability & small Stokes-shift

• Especially suited for Argon-Ion & Nd: YAG laser

 

Description

Protein

 

Atto532-Actin

Origin

 

Alpha cardiac actin, bovine

Molecular mass

 

42kDa

Protein description

 

 

 

 

 

Actin from bovine cardiac muscle is a single chain polypeptide with a molecular

weight of 42kD consisting of 375 amino acids. Atto532-Actin is chemically modified

G-actin with an Atto532 fluorescent group covalently bound to lysine residues.

Atto532-Actin (λem 532nm, λem 553nm) possesses the polymerization properties

of native G-actin.

Conjugation ratio

 

 

 

This product is supplied with a degree of labelling (DOL) between 0.5 and 0.8.

The precise DOL is stated in the DataSheet supplied with the product.

 

Properties

Form

 

Lyophilized, ready-to-use

Quantity

 

1x100 µg, 5x100 µg

Buffer

 

 

 

 

2mM Tris-Cl pH 8.2, 0.4mM ATP, 0.5mM DTT, 0.1mM CaCl2, 1mM NaN3 and 0.3%

disaccharides, when reconstituted with 1.0 ml ultrapure water to obtain a 1mg/ml

solution.

 

Purity

 

 

>99% by scanning densitometry

 

Purification notes

 

 

Purified from bovine cardiac muscle.

 

Protein conc.

 

 

Determined by the Biuret method.

 

Storage instructions

 

 

 

Atto-Actins are stored at -70°C upon arrival will be stable in performance for

at least 6 months from the date of purchase. Solubilized Atto-Actin is kept on

ice and should be used within 1 week. Avoid refreezing.

 

Shipping conditions

 

 

At ambient temperature. Upon delivery store at -70°C.

 

 

Ordering informations

 

Catalog No.

Product Name

Size

8252

Atto390-Actin (alpha-cardiac actin, bovine)

1 x 100 µg, 5 x 100 µg

8255

Atto532-Actin (alpha-cardiac actin, bovine)

1 x 100 µg, 5 x 100 µg

 

 

▣ 관련 페이지 ; HYPERMOL EK

 

  

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